以子叶全展期的棉花幼苗为材料，在1/4 Hoagland营养液中添加不同浓度，0，10，50，100，200μmol/L SNP （硝普钠）处理棉花幼苗，研究外源NO对棉花幼苗叶片和根系氧化损伤和保护酶活性的影响。结果表明：外源NO供体SNP处理使棉苗叶片和根系MDA含量和O·-2产生速率显著增加，可溶性蛋白含量（叶片）显著降低，SOD、POD活性增加，但是CAT活性下降。10～100μmol/L SNP处理间差异不显著。各生理指标之间差异优先表现在叶片中。结果表明，外源NO在一定浓度范围内抑制棉花幼苗的生长。%Cotyledon full extension of the cotton seedling as material ,in the 1/4 Hoagland nutrient solution with different concentration of SNP( 0,10,50,100,200 μmol/L)processing cotton seedling,study of oxidative damage and activities of protective enzymes of exogenous nitric oxide on cotton seedling leaves and roots .The results show that:with the treatment of SNP ,the content of MDA and the generation rate of O ·-2 of cotton seedling leaves and roots increased significantly ,the content of soluble protein of leaves decreased significantly , SOD and POD activity in-creased ,while CAT activity decreased .The difference under 10-100 μmol/L SNP processing were not remarkable between treatments .The difference of physiological indexes between treatments was priority performance in leaves . These results suggest that exogenous nitric oxide in certain concentration range inhibit cotton seedling growth .
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