玉米自交系 AS－9化学诱变后代 SSR 遗传变异分析

摘要

为分析系列化学诱变对玉米自交系 AS－9诱变后代的影响，以玉米自交系 AS－9及其化学诱变自交第1代M1和第4代 M4为材料，进行发芽试验，同时利用分布在10条染色体上的68对 SSR 引物进行遗传变异分析。结果表明：诱变 M1和 M4种子的发芽率、芽长和根数普遍高于对照；能在2代玉米试材中扩增出多态性条带的引物有54对，占引物总数的79．41％；M1诱变系与基础材料对照的遗传相似系数平均值为0．3647，遗传相似系数中心化后的数据在－0．12处，可将材料分为2个类群，基础材料（ⅠCK）为一类，诱变系为一类； M4诱变系与基础材料对照的遗传相似系数平均值为0．4346，遗传相似系数中心化后的数据在－0．09处，可将材料分为基础自交系（ⅣCK）和诱变系2个类群；M1和 M4主成分分析与聚类结果基本一致。说明基础材料 AS－9与诱变系材料间存在真实的遗传差异，系列化学诱变已使玉米自交系 AS－9产生了广泛的遗传变异。%　　Analysis of genetic diversity in maize germplasm is of great importance to conserving and utilizing these valuable chemical mutagenic resources .In this research,the genetic differences between mutants (M1&M4) and the corresponding orginal material (AS-9) was studied based on SSR markers with selected 54 polymorphism SSR primers.The results showed that the seed germination rate ,bud length and root number of M1 &M4 were higher than AS-9 in most cases.In two generations maize materials,54 polymorphism primers can be amplified,accounting for 79.41% of the total.Relatived toⅠCK ,the average value of genetic similarity coefficient of M1 was 0.364 7. With the certered genetic similarity coefficient of -0.12 by UPGMA,the maize inbred line materials were divided into two groups:the orginal material(ⅠCK) and mutants.Relatived to ⅣCK,the average value of genetic similarity coefficient of M4 was 0.434 6.With the cemtered genetic similarity coefficient of -0.09,the maize materials were classified into two groups:ⅣCK and mutants.Principal component analysis and clustering results were similar .The results showed there were distinct variation between orginal material AS -9 and induced material,indicating chemical mutagen had produced genetic variations in maize inbred lines on large scale .