高温条件下CO2对黄瓜SOD、POD和CAT活性及其基因表达的影响

摘要

In order to explore the effect of CO2 on SOD, POD and CAT activity and their gene expressions under high temperature, the grafting cucumber in greenhouse was used as material, trying to study the changes of cucumber leaf SOD, POD and CAT activity and gene expression under high temperature after treating with CO2.The results showed SOD,POD and CAT activity in cucumber leaf were increased after treating with CO2 under high temperature.Comparing with room temperature treatment,the max difference of cucumber SOD,POD and CAT activity were at 42,28 and 21 days after treating with CO2 and high temperature,and increased by 19.1%,50.4% and 45.0% respectively.Using Real-time PCR analysis cucumber SOD,POD and CAT gene expression were all increased after treating with CO2 under high temperature.The max difference of cucumber SOD,POD and CAT gene expression level were at 28,28 and 21 days after treating with CO2 and high temperature,and with 60.7%,70.3% and 44.9% higher than room temperature treatment respectively.In conclusion,after treating with CO2,the cucumber SOD,POD and CAT activity and their gene expression level were both increased under high temperature.%为了探索高温条件下,CO2对保护酶SOD、POD和CAT活性及其基因相对表达量的影响,以温室嫁接黄瓜为材料,研究高温条件下,CO2处理后黄瓜叶片中SOD、POD和CAT活性及其基因相对表达量的变化趋势.结果显示,高温条件下,CO2处理增加了黄瓜叶片SOD、POD和CAT的活性.与常温处理相比,高温结合CO2处理黄瓜的SOD、POD和CAT活性分别在处理42,28,21 d时差异最大,分别增加了19.1%,50.4%和45.0%.利用Real-time PCR分析SOD、POD和CAT基因相对表达量,结果显示,高温条件下,CO2处理增加了黄瓜叶片的SOD、POD和CAT基因的相对表达量. 高温结合CO2处理中黄瓜的SOD、POD和CAT基因相对表达量分别在处理28,28,21 d时达到最大值,比常温处理增加了60.7%,70.3%和44.9%.结果表明,高温结合CO2处理增加了温室黄瓜SOD、POD和CAT的活性及其基因的表达水平.