宜昌百合体细胞胚诱导及植株再生

摘要

The objective of this paper was to preserve and propagate the wild resources and to establish a simplest and efficient method for high frequency of somatic embryogenesis and plant regeneration of Lilium leucanthum(Baker). The effects of hormone combination, different places in scales and the condition of light or dark on inducing somatic embryogenesis were studied. The results showed that the appropriate medium of the somatic embryogenesis induced from scales was MS + 0. 5 mg/L TDZ + 1. 0 mg/L NAA. The induction rate of somatic embryogenesis of scales at base was 90. 00% which was the highest in the three parts of explants. The ability of scale parts inducing somatic embryogenesis was lower > middle > upper. The induction rate of somatic embryogenesis under the dark condition was 90. 00%, which was higher than the light condition.%为了对秦岭野生宜昌百合资源进行保存,并有效地解决其扩繁和再生问题,以秦岭野生宜昌百合为材料,研究不同质量浓度的激素组合、鳞片的不同部位及光暗培养对秦岭野生宜昌百合的体细胞胚诱导的影响.结果表明,鳞片诱导体细胞胚的适宜培养基为MS+0.5 mg/L TDZ(N-苯基-N′-1,2,3-噻二唑-5-脲)+1.0 mg/L NAA(a-萘乙酸).鳞片基部诱导体胚率最高,为90.00％,鳞片分化体细胞胚的能力为基部＞中部＞上部.暗培养下诱导率为90.00％,高于光培养条件.