纤维素降解真菌的筛选与鉴定

摘要

A high-efficiency cellulose-degrading fungus, designated as C006, was isolated from field soil samples and rotten wood samples in original forest in Qinling moutain through cellulose medium (CM) with crystalline cellulose as the sole carbon source. The strain was identified as Trichoderma atroviride based on ITS rDNA sequence and phylogenetic tree and morphological characteristics. The degradability of strain C006 to crystalline cellulose was confirmed through congo-red stanning, a transparent degrading-halo appeared on the CM medium and liquid CM medium. Great amount of acidic substances were also produced during crystalline cellulose degradation by the strain C006.%通过对采集于农田的土壤及秦岭原始森林中的腐木样本进行微生物富集培养,以微晶纤维素为唯一碳源进行筛选、分离和纯化,获得一株可利用微晶纤维素的真菌C006,依据ITS rDNA测序及聚类分析并结合形态学特征,鉴定为深绿木霉(Trichoderma atroviride).经液体发酵、固体平板培养及刚果红染色,进一步确认C006菌株对微晶纤维素有较强的降解能力.同时,还发现C006菌株在利用微晶纤维素时产生大量的酸性物质.