为筛选较适合的酒酒球菌膜脂肪酸提取和皂化方法,采用5种不同脂肪酸分析样品前处理方法,结合气相色谱质谱联用仪(GC/MS),分析酒酒球菌SD-2a膜脂肪酸成分,比较不同处理方法的效果.结果表明,先提取脂肪酸再采用NaOCH3甲酯化脂肪酸的方法能够较为准确和完整地检出酒酒球菌SD-2a的12种膜脂肪酸,相对标准误差(RSD)≤5％,特别对于环丙烷脂肪酸的检测效果较好,RSD=2％；独立的脂肪酸提取步骤对细菌膜脂肪酸分析是必要的,包含脂肪酸提取的样品处理方法比不包含脂肪酸提取的方法多检出3种脂肪酸,RSD均≤5％.%Five different methods combined with GC/MS analysis of membrane fatty acid composition of Oenococcus oeni SD-2a were compared. This study validated that the method including lipids extraction and NaOCH3 methylation was more accurate and efficient, by which 12 fatty acids were detected, RSD is less than 5 %, and it was suitable particularly for analysis of cyclopropane fatty acids such as C19cycll fatty acid, with a 2% RSD;the study also indicated that the lipids extraction procedure prior to the methylation of samples cannot be omitted. Three more fatty acids, in which RSD were all less than 5 %, were detected in the method involving lipids extraction procedure than the method without lipids extraction.
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