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超干处理对柳枝稷种子活力及幼苗生长特性的影响

     

摘要

研究超干处理对柳枝稷(Panicum virgatum L.)种子活力及幼苗生长特性的影响,为柳枝稷种子耐干性及贮藏提供理论基础.采用硅胶干燥法将柳枝稷种子含水量由9%分别降至7%,5%,3%,2%,1%,测定不同含水量条件下种子的活力以及种子萌发后第14 d幼苗的叶面积、根长、苗长、根冠比、鲜重、干重及相对电导率等指标的影响.柳枝稷种子含水量由对照9%降到2%的过程中,种子仍保持较高活力.含水量降至1%种子活力显著下降,但种子的相对电导率与种子活力没有显著相关性.超干处理后幼苗生长正常.柳枝稷种子属于正常型种子,其贮藏的安全水分下限为2%.超干处理并没有破坏柳枝稷种子细胞膜的完整性,也未对幼苗的生长造成损伤.%In order to provide a theoretical basis for desiccation tolerance and the storage of switchgrass (Panicum virgatum L.) seeds, we investigated the effect of ultra-dry treatment on the vigor of switchgrass seeds and seedling growth.The moisture content of switchgrass seeds was reduced to 7 %, 5 %, 4 %,3%, 2% and 1% from 9% (control), respectively, with silica gel, and the vigor were detected when the seeds were re-moistened.And the leaf area, root length, seedling length, root/shoot ratio, fresh weight,dry weight and relative electrical conductivity were detected after the seed had germinated in 14 days.In the process of reducing the moisture content of switchgrass seeds from 9 % to 2 %, the seed vigor was kept relative high.When the moisture was reduced to 1%, seed vigor decreased significantly.However, there was no significant difference between seed vigor and relative electrical conductivity.The seedling growth of the ultra-dry seeds was normal.Switchgrass seed was an orthodox seed and its low-limit of safe moisture content was 2%.Ultra-dry treatment didn't damage the integrity of the cell membrane and the seedling growth.

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