We studied optimal conditions for the determination of oxygen consumption rate (OCR).The assay, with a working range up to 35 mmol/L, had an intra-assay coefficient of variation of 0. 7%～3. 52%, and the inter-assay coefficient of variation was 4. 86%. In spiking experiments, the recovery range was 98. 9%～101 %. The assay was compared with CX3 (Beckman Co.). A linear regression analysis yielded a slope of 1. 05, an inercept of 3. 82 and a correlation coefficient of 0. 999. The serum of turbidity above + + had a negative interference on the assay (>3. 4%). The obvious interference of hemolysis, icterus and vitamin C to the assay were not observed.
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