小鼠睾丸曲细精管生精上皮的分离及形态观察

摘要

Objective To explore a simple and feasible technique to locate proteins during spermato-genesis. Methods Various germ cells and somatic cells were separated by collagenase I and Dnase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and con-focal microscopy. Results Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus. Conclusion A simple method for locating proteins during permatogenesis was successfully developed.%目的 寻找一种简单可行的研究精子发生过程中蛋白质定位的方法.方法 将新鲜的小鼠睾丸去除白膜,通过胶原酶Ⅰ及DNA酶Ⅰ的作用分离各种细胞.细胞经简单处理后以合适的浓度铺片,进行荧光染色,并于共聚焦荧光显微镜下观察.结果 通过分析特异的染料对顶体和细胞核染色的结果,可以分辨出各个阶段的生精细胞.结论 建立了一种研究精子发生过程中蛋白质定位的简单、可靠的方法.