Objective To study the effect of +Gz stress on testosterone (T) and cortisol (C) levels and T/C in saliva of pilots. Methods Forty-seven pilots of high performancefighter were included in this study. Saliva samples were taken before and after they were exposed to +6.5 Gz, +7.0 Gz and +8.0 Gz. T and C levels in saliva were measured by chemiluminescent immunoassay. Results Nosignificant difference was found in the T level in saliva of the pilots before and after exposure to +6.5 Gz, +7.0 Gz and +8.0 Gz. The C level was significantly higher in saliva of the pilots after exposure to +6.5 Gz, +7.0 Gz and +8.0 Gz than before exposure to them (P<0.05) and after exposure to +8.0 Gz than before exposure to +6.5 Gz, +7.0 Gz and +8.0 Gz (P<0.05). No significant difference was found in the T/C in saliva ofthe pilots after exposure to different +Gz stresses although it tended to change. ANOVA analysis showed that the type offighter, the age and totalflight time of pilots had no effect on the T and C levels and T/C after exposure to different +Gz stresses. The T level was not related with the C level in saliva of pilots after exposure to different +Gz stresses. Age was positively related with baseline T/C.Conclusion The elevated C level in saliva of pilots after exposure to +Gz indicates that saliva can be used as an indicator of stress and fatigue in pilots.%目的：探讨高正加速度(+Gzz)应激对飞行员唾液睾酮(testosterone，T)、皮质醇(cortisol，C)及二者比值(T/C)的影响。方法47例高性能战斗机飞行员为研究对象，分别在+6.5 Gz，+7.0 Gz和+8.0 Gz暴露前后取受试者唾液，采用微粒子化学发光法检测唾液T及C水平。结果+6.5 Gz、+7.0 Gz、+8.0 Gz作用后唾液T浓度与暴露前相比无统计学差异；唾液C的浓度均高于+Gz暴露前水平(P＜0.05)，其中+8.0 Gz暴露后C较+6.5 Gz后、+7.0 Gz前、+8.0 Gz前均高(P＜0.05)；T/C值在不同+Gz暴露后呈现出不同的变化趋势，但并没有统计学差异。多元方差分析结果显示机种、年龄、飞行总时间对不同+Gz暴露后飞行员唾液T、C以及T/C变化无影响。唾液T与C在高+Gz应激后的变化无相关性；年龄与基础T/C呈正相关性。结论+Gz暴露时提高了飞行员的唾液C浓度，提示唾液可能作为飞行员应激及疲劳的评价指标。
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