Objective To study the effect of zinc on expression of substance P in mice with sciatic nerve injury (SNI). Methods Forty-eight CD1 mice were divided into control group, neuropathic pain (NPP) group, low NPP (L-NPP) group, and high NPP (H-NPP) group. A NPP model of mice was induced by surgery for SNI. Mice in control group underwent sham operation (isolating sciatic nerve) while those in N-NPP group, L-NPP group and H-NPP group underwent surgery for SNI after they were fed with forage containing zinc at the dose of 30 mg/(kg · d) and 0.85 mg/(kg · d) and with zinc sulfate water solution at the concentration of 227 mg/(L · d) respectively for 2 weeks. Effect of zinc on expression of substance P in mice with SNI was detected by atomic absorption spectrum, immunohistochemistry, immune blotting and image analysis respectively on day 7 after operation. Results The serum zinc level in spinal cord was significantly higher and the substance P expression level was significantly lower in H-NPP group than in L-NPP group (P<0.01).Conclusion Zinc can inhibit the expression of substance P in mice with SNI.% 目的研究锌对选择性坐骨神经分支损伤(spared nerve injury，SNI)小鼠脊髓P物质(substance P，SP)表达的影响。方法48只CD1小鼠分为4组，采用SNI手术制备神经病理性疼痛(neuropathic pain，NPP)小鼠模型：对照组(Con)给予假手术(单纯分离坐骨神经)，适锌喂养神经分支损伤(N-NPP)组锌饲料30 mg/(kg·d)喂养2周后给予SNI手术，低锌喂养神经分支损伤(L-NPP)组锌饲料0.85 mg/(kg·d)喂养2周后给予SNI手术，高锌喂养神经分支损伤(H-NPP)组硫酸锌水溶液227 mg/(L·d)喂养2周后给予SNI手术。应用原子吸收光谱、免疫组织化学、免疫印迹和图像分析技术检测术后7d锌对脊髓SP表达的影响。结果高锌喂养能显著增加血清和脊髓中锌的含量，脊髓SP表达下调(P＜0.01)；而低锌喂养加重血清和脊髓的缺锌状况，脊髓SP表达上调(P＜0.01)。结论锌能抑制SNI后小鼠脊髓SP的表达。
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