水通道蛋白4基因表达与阿尔茨海默病相关性的动物实验

摘要

Objective To explore the correlation between aquaporin 4 (AQP4) and Alzheimer's disease (AD).MethodsAgilent expressionprofile chip was used to screen the gene expression differences through the comparison between SAMP8 and SAMR1 after total RNA of brain tissue being extracted out, then Real-Time PCR technology and indirect immunofluorescence assay were applied for identification.ResultsTotally 804 genes related to dementia were selected, among which the number of up-regulated genes was 521 and down-regulated genes was 283. The biological function and pathways of the related genes were searched in GeneBank, then six genes were found to be closely related to AD: Herc6, Lyst, Nkain1, Aqp4, Uch13, Slc9a8. The fold change of Aqp4 was 6.26 withP<0.001. The Real-time PCR of the Aqp4 gene showed that the expression quantity in SAMP8 group was higher than that in SAMR1 group (P= 0.037 6), and the immunofluorescence assayshowed that the expression of Aqp4 in SAMP8 also increased.Conclusion Increased Aqp4 expression is related to the increased need for Aβ clearance in SAMP8 rats which has astrocytes compensatory proliferation.%目的：探讨水通道蛋白-4(aquaporin 4，AQP4)基因表达与阿尔茨海默病的相关性。方法应用Agilent表达谱芯片，选用快速老化痴呆模型小鼠(SAMP8)，通过与同月龄正常对照小鼠(SAMR1)比较，提取脑组织总RNA，筛选出差异基因并应用Real time-PCR技术和免疫荧光方法进行鉴定。结果筛选差异表达基因共804个，其中表达上调521个，表达下调283个，在GeneBank查找相关基因的生物学功能及通路，找到与AD密切相关基因有6条：Herc6，Lyst，Nkain1，Aqp4， Uch13，Slc9a8，其中Aqp4基因的差异倍数为6.26(P＜0.001)。Real time-PCR检测结果显示，SAMP8组Aqp4基因表达量较SAMR1组增高(P=0.0376)，免疫荧光提示SAMP8组中Aqp4表达增加。结论 Aqp4在SAMP8小鼠中表达量增多，可能与清除β淀粉样蛋白沉积、代偿性星形胶质细胞增生有关。