While traditional in vitro models provide useful information in biomedical and pharmaceutical research, they often fail the translation to relevant results. This failure occurs mainly because of the diminished ability of the 2D cell cultures to replicate the microanatomy and physiology of its original tissues. 3D organoid technology mitigates many of the spatial and anatomic limitations of 2D culture. Standardization of isolation, culture, and harvesting of canine intestinal and hepatic organoids has been developed as a necessary basic feature for practical use of the technology. Furthermore, the methods of seeding dual-chamber permeable support system, the organoid monolayer maintenance and the experimental settings have been developed and described. A successful permeability study was performed exploring the intestinal passive permeability of beta-blockers (propranolol, metoprolol, and atenolol) through intestinal barrier. The results of the experiment were compared to traditional 2D Caco-2 cell model. This experiment serves as a proof-of-concept study providing valuable results supporting canine organoid monolayers as a tool for species-specific drug permeability studies. Additionally, to the canine intestinal and hepatic organoids, five more canine organoid systems derived from different organs (bladder, endometrium, kidney, lung, and pancreas) have been developed and described. These inventions improve on the usability of the canine organoid technology in biomedical research and paraclinical applications. Finally, media components driving differentiation of cholangiocytes to hepatocytes in the canine liver organoids were explored and most useful media compositions were identified.
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