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Ruminant respiratory syncytial virus subgroup distiction using synthetic peptides from the extracellular central region of the attachment protein -G.

机译:反刍呼吸道合胞病毒亚组分布,使用来自附着蛋白-G胞外中央区域的合成肽。

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摘要

Subgroup specific, peptide-based, enzyme immunoassays were developed from the unique extracellular, central region (residues 158--189) located between two mucin-like regions of the attachment protein-G from ovine and bovine respiratory syncytial viruses. Antigenic peptides [ovine (residues 173--189) and bovine (residues 171--187)] used to develop the enzyme immunoassays were identified by a combination of algorithms and epitope mapping from each G-glycoprotein. The negative threshold for each enzyme immunoassay was established as the mean optical density of indirect immunofluorescent antibody-negative bovine sera plus three standard deviations. The sensitivity, specificity, positive and negative predictive values of each enzyme immunoassay was determined by comparison with indirect immunofluorescence (gold standard). Antibody prevalence to the ovine and bovine subgroups of respiratory syncytial virus infections was determined from 1,102 bovine serum samples obtained from six diagnostic laboratories from the Northwest and Southeast United States. The Southeast had a higher prevalence of the bovine subgroup strains (69.5%) [compared to the Northwest (40.9%)], whereas the prevalence of the ovine strain was similar for the two regions [Southeast with 16.7% compared to the Northwest with 14.9%]. The overall prevalence was 56.6% for the bovine strain and 15.9% for the ovine strain. The immunoassays then were used to determine the prevalence of ruminant RSV subgroups in a herd of Rocky Mountain Bighorn Sheep. Antibody to the ovine-specific peptide was found in 68% of samples tested whereas 28% of serum samples reacted with the bovine-specific peptide. Results indicated that viruses from both subgroups (ovine and bovine) of ruminant respiratory syncytial virus infect bighorn sheep. The implications of all results were discussed.
机译:从位于绵羊和牛呼吸道合胞病毒的附着蛋白G的两个粘蛋白样区域之间的独特细胞外中心区域(残基158--189)开发了基于亚组的,基于肽的酶免疫测定法。通过算法和每种G-糖蛋白的抗原决定簇结合,鉴定了用于进行酶免疫测定的抗原肽[绵羊(173--189)和牛(171--187)]。将每种酶免疫测定的阴性阈值确定为间接免疫荧光抗体阴性牛血清的平均光密度加三个标准偏差。通过与间接免疫荧光法(金标准)比较,确定每种酶免疫测定法的灵敏度,特异性,阳性和阴性预测值。从美国西北和东南部的六个诊断实验室获得的1,102份牛血清样本中确定了呼吸道合胞病毒感染的绵羊和牛亚群的抗体流行率。东南部的牛亚种菌株的患病率较高(69.5%)[相比西北地区的(40.9%)],而这两个地区的绵羊品系的患病率相似[东南部的16.7%,西北部的14.9 %]。牛品系的总患病率为56.6%,羊品系的总患病率为15.9%。然后使用免疫测定法确定落基山大角羊群中反刍动物RSV亚群的流行程度。在68%的待测样品中发现了针对绵羊特异性肽的抗体,而28%的血清样品与牛特异性肽发生了反应。结果表明,反刍动物呼吸道合胞病毒的两个亚组(绵羊和牛)的病毒都感染了大角羊。讨论了所有结果的含义。

著录项

  • 作者

    Grubbs, Steven Timothy.;

  • 作者单位

    The University of Tennessee.;

  • 授予单位 The University of Tennessee.;
  • 学科 Biology Microbiology.;Health Sciences Immunology.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 184 p.
  • 总页数 184
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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