Infrared signatures of mammalian cells in culture.

摘要

The mineralization behavior of the clonal osteogenic cell line, MC3T3-E1 cells, was mostly studied by conventional biochemical and microscopic analysis methods in the tissue culture polystyrene system. In this thesis, the mineralization behavior of MC3T3-E1 cells cultured in both the rotary tissue culture system and the tissue culture polystyrene system was investigated by the FTIR/transmission technique, together with the in-phase and/or phase contrast microscopy technique. The second derivative FTIR spectra indicated that calcified mineral HA was formed at day 21 in the tissue culture polystyrene system, and at day 4 in the rotary tissue culture system when cells were fed by the modified medium. In contrast, there was no evidence showing the formation of HA in the tissue culture polystyrene system, or the formation of HA was unstable and disappeared at day 13 in the rotary tissue culture system when cells were fed by the controlled medium. It was suggested that the rotary tissue culture system accelerated the mineralization process of MC3T3-E1 cells, and L-ascorbic acid and beta-glycerophosphate were required for HA formation.