In-vitro hypocholesterolemic effect of multiple bacteria probiotics combined with levan fructo-oligosaccharide.

摘要

Increasing health concern and economic implications of high cholesterol is triggering a shift in selective choice of food that contains less fat and cholesterol. The aim of this study was to determine the ability of Lactobacillus acidophilus, L. reuteri and Bifidobacterium longum combined with the fructo-oligossaccharide Levan to consume cholesterol in deMan, Rogosa and Sharpe (MRS) broth. To the MRS broth Lactobacillus acidophilus and Lactobacillus reuteri were added individually and in multiple combinations and they were cultured at 20 hrs and 24hrs at 37oc anaerobically in the presence of 0, 0.3 and 0.5% oxgall to assay the cholesterol uptake. Bifidobacterium longum was incubated at 48hrs and 72hrs in MRS added with 0.05% L-cysteine hydrochloride at 37oc at 0, 0.3, 0.5% oxgall. The uptake of cholesterol by L. acidophilus, L. reuteri and Bifidobacterium longum at 0,0.3 and 0.5% ox-gall concentrations and 20, 24 and 48 hrs. Incubation was 44.24, 36.3 and 31.6 % , 62.87, 42.12 and 24.11%, and 29.13, 36.23 and 37.44, respectively. These findings demonstrate that although there was a marked reduction in coliform forming units associated with higher concentrations of bile or oxgall, these high concentrations of bile were also associated with an increase in clearance of cholesterol in the MRS media by these microbes, especially Bifidobacterium. Also, Levan from Bifidobacterium was successfully produce, characterized and evaluated for its efficacy in cholesterol reduction in-vivo. In this study the effect of Levan a fructo-oligosaccharide from Lactobacillus reuteri also significantly reduced cholesterol in-vivo with and without bile.