List of key abbreviations
1. Introduction
1.1 Research background and significance
1.2 Research contents
1.3 Research objective
1.4 Technical route
2. Literature review
2.1 The theoretical basis of the composting process
2.2 Technology of composting process
2.3 Microbiological aspects of composting process
2.4 The phenomenon of microbial uncultivability
2.5 Resuscitation-promoting factor
3. Materials and methods
3.1 Production of recombinant Rpf protein
3.2 Soil sample collection and isolation of bacteria
3.3 Identification of bacteria by sequencing of the 16s rRNA and phylogenetic analysis
3.4 Investigation of organic substances hydrolytic capabilities of VBNC bacteria
3.5 Study on the effect of Rpf on cellulose degradation in composting
4. Results and discussions
4.1 Effect of Rpf and analysis of cell growth
4.2 Physicochemical characteristics of soil sample
4.3 Screening the strains
4.4 Sequence analysis of 16S rRNA gene and the phylogenetic relationship
4.5 Resuscitation of VBNC bacteria for enhanced cellulose degradation in composting
5.1 Research conclusion
5.2 Main innovation points
5.3 Research outlooks
参考文献
声明
浙江师范大学;
