声明
致谢
Chapter 1:Introduction
Background
1.1.Gold nanoparticles and their antibacterial propensity
1.1.1.Gold nanoparticles and their properties
1.1.2.Antimicrobial propensity
1.2.Biological synthesis of multifunctional gold nanoparticles, their powerful catalytic, and anti-oxidant properties
1.2.1.Biological synthesis of gold nanoparticles
1.2.2.Synthesis of gold nanoparticles using microorganisms
1.2.3.Biologically produced gold nanoparticles with antioxidant and catalytic potential
1.3.Functionalization and conjugation of antimicrobial peptides with metal-based nanoparticles
1.3.1.Post-synthesis and functionalization of metal-based nanoparticles, non-toxic ways for the synthesis of metal-based nanoparticles with applications
1.3.2.Antimicrobial peptideswith metal-based nanoparticles
1.4.Aims, objectives,and outline of this dissertation
1.4.1.Aims and objectives
1.4.2 Dissertation outline
1.4.3 Outline of the author’s contributiontothis dissertation
Chapter2: Shape-dependent significant physical mutilation and antibacterial mechanisms of gold nanoparticles against foodborne Gram-positive and Gram-negative pathogenic bacteria at lower concentrations
Background
2.1.Materials and methods
2.1.1. Materials
2.1.2. Synthesis of gold nanoparticles
2.1.3. Characterization of gold nanoparticles
2.1.4. Bacteria and growth conditions
2.1.5. Disk diffusion test(Qualitative analysis)
2.1.6. Killing kinetics of gold nanoparticles(Quantitative analysis)
2.1.7. Microscopic study(Visual analysis)
2.1.8. Nucleicacid leakage
2.1.9. Statistical analysis
2.2. Results and discussion
2.2.1. Characterization
2.2.2. Antibacterial activity of AuNPs against foodborne bacterial pathogens
2.2.3. Antibacterial mechanisms of AuNPs against foodborne bacterial pathogens
2.3. Conclusion
2.4. Essential supplementary materials
Chapter 3: Extracellular synthesis of multifunctional gold nanoparticles by Lactobacillus acidophilus with powerful catalytic, electrochemical sensing, anti-oxidant and antibacterial properties
Background
3.1. Materials and methods
3.1.1. Materials
3.1.2. Bacterial growth and maintenance
3.1.3. Biosynthesis of AuNPs
3.1.4. Characterization techniques
3.1.5. Antioxidant property
3.1.6. Catalyticactivity
3.1.7. Antibacterial sensitivity test
3.1.8. Statistical analysis
3.2. Results and discussion
3.2.1. Extracellular synthesis of AuNPs
3.2.2. Physicochemical characterization
3.2.3. AuNPs as an electrochemical sensor
3.2.4. AuNPs as a potential antioxidant
3.2.5. AuNPs as apotential catalyst
3.2.6. Antibacterial activity of AuNPs against Gram-positive and Gram-negative bacteria
3.3. Conclusion
3.4. Essential supplementary materials
Chapter 4: Improved antibacterial activity of nisin in-conjunction with metallic and bimetallic nanocomposites of gold and silver produced by Lactobacillus acidophilus
Background
4.1. Materials and methods
4.1.1. Materials
4.1.2. Bacterial growth and maintenance
4.1.3. Biological synthesis of AuNPs, AgNPs, and AuAgNCs using L. acidophilus
4.1.4. Nanoconjugation strategy for AuNPs, AgNPs, and AuAgNCs with nisin
4.1.5. Characterization techniques
4.1.6. Antibacterial activity of nisin and nanocomposites before and after conjugation
4.1.7. Statistical analysis
4.2. Results and discussion
4.2.1. Synthesis and characterization of AuNPs, AgNPs, and AuAgNCs
4.2.2. Nanoconjugation of AuNPs, AgNPs, and AuAgNCs with nisin and their characterization
4.2.3. Enhanced antibacterial activity of nisin and nanocomposites before and after conjugation
4.3. Conclusion
4.4. Essential supplementary materials
Chapter 5: General conclusions and future prospective
5.1. Conclusions
5.1.1.Conclusions on shape-dependent antibacterial activity of gold nanoparticles against foodborne pathogenic bacteria
5.1.2.Conclusions on biological synthesis of gold nanoparticles by Lactobacillus acidophilusand their potential properties
5.1.3.Conclusions onimproved antibacterial activity of nisin in-conjunction with metallic and bimetallic nanocomposites of gold and silver produced by Lactobacillus acidophilus
5.2.Future prospective
参考文献
Appendix
A 1. Results
A 1.2. Synthesis of AuNPs, AgNPs, and AuAgNCs with different ratios of AgNO3 and HAuCl4 using Lactobacillus acidophilus
A 1.3.Synthesis of AuNPs-nisin,AgNPs-nisin, and AuAgNCs-nisin conjugates from freshly prepared AuNPs, AgNPs, and AuAgNCs (with different ratios of AgNO3 and HAuCl4)
A 2. Author’s contribution to research study during Ph. D degree
A 2.2. Shape-dependent significant physical mutilation and antibacterial mechanisms of gold nanoparticles against foodborne bacterial pathogens (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) at lower concentrations. Materials Science
A 3.Author’s biography
浙江大学;
