Hydrolysis of Bamboo Cellulose by Cellulases from Marine-derived Fungi Strains

摘要

Penicilium imranainum是来自于海洋的耐盐性真菌，本文研究了这种真菌在液态发酵的条件下产纤维素酶的特性。对真菌培养参数（盐度、pH和温度）以及真菌所产纤维素酶对竹纤维的降解特性进行了系统的研究，并且和常见的产酶真菌 Aspergillus niger进行对比。结果表明：液态发酵条件下所有菌株产酶的最佳培养时间是5天，而滤纸酶活性最高的培养时间是7天。通过优化pH和温度，两种真菌所产纤维素酶的对滤纸的活性分别是1.9和2.8FPU g-1。P. imranianum和A. Niger.所产纤维素酶对纤维素的糖化率分别是81%和56%。本研究还以慈竹作为研究对象，发现两种真菌酶对未经预处理的竹子不具备降解活性。而竹子经过预处理后，P.imranianum和A. niger.分别获得了81%和58%的糖化率。相比较于A. Niger.，P. imranianum更具水解纤维素潜力。同时，P. imranianum酶显示了在极端条件下工作的特性，该真菌所产酶可以在pH为9，盐度10%，温度为60℃的时候正常工作。在生物质燃料的生产中显示出良好的应用前景。

目录

声明

1 Introduction

1.1 Background

1.2 Extremophile

1.3 Halophile

1.4 Bamboo

1.5Biochemical conversion

1.6 Literature review

1.7 Research content

1.8Features and Innovations

2. Material and Method

2.1 Experiment design and research plan

2.2 Pretreatment

2.2 Composition of Bamboo and Extract Analysis

2.3 Fungi strains and inoculum preparation

2.4 Enzyme extraction

2.5 Enzyme activity measurements

2.6 Optimization of enzymatic hydrolysis conditions on the enzyme assay

2.7Statistical analyses

3. Results

3.1 Pretreatment

3.2Calculation for unknown time

3.3Solid Substrate Fermentation(SSF)

3.4Submerged Fermentation

3.5 Growth on pure Bamboo Solution

3.6 CMcase

3.7 FPASE

3.8 Calculation of change inmedia

3.9 Change in Temperature

3.10 Effect of Salinity

3.11 Saccharification of Bamboo

4. Conclusion

5. Discussion

参考文献

致谢