声明
Abbreviation
Chapter1 Introduction
1.1 Oxidative stress in plants: Case of Arabidopsis thaliana
1.1.1 Arabidopsis thaliana: Overview
1.1.2 Oxidative stress in plants: Arabidopsis thaliana
1.1.3 Reactive oxygen species (ROS) and their role in oxidative stress in A. thaliana
1.2 Sulfiredoxin
1.2.1 Structure of Sulfiredoxin
1.2.2 Functions of Sulfiredoxin in A. thaliana
1.2.3 Mutational variation of sulfiredoxin and their functions
Chapter2 Materials and Methods
2.1 Experimental materials
2.1.1 Experimental strains and vectors
2.1.2 Main reagents in the experiment
2.1.3 Buffer and reagent preparation
2.1.4 Laboratory instruments and equipment
2.2 Clone construction
2.2.1 Amplification of target fragment by PCR
2.2.2 Double digestion of PCR products and vectors
2.2.3 Ligation reaction
2.2.4 Transformation of E. coli competent cells
2.2.5 Recombination verification test
2.3 Protein expression and purification
2.3.2 Small-scale protein expression
2.3.3 Large-scale protein
2.3.4 Purification of protein by affinity chromatography
2.3.5 Purification of protein by gel filtration chromatography
2.4 Crystal Screen
Chapter3 Results
3.1 Clone construction
3.2 Recombined protein expression and purification
3.3 Crystal growth conditions of Srx
Chapter4 Conclusion, discussion and prospect
4.1 Experimental conclusion
4.2 Discussion of recombinant protein Srx expressed on different vectors
4.3 Discussion on the interaction between Srx and Prx
4.4 Prospect of sulfiredoxin
参考文献
Appendix
致谢
安徽大学;
