声明
摘要
Abstract
Table of Contents
Chapter 1 Introduction
1．1．1 Cardiovascular disease，atherosclerosis
1．1．2 Coronary artery bypass graft surgery and vascular grafts
1．1．3 Percutaneous coronary intervention and coronary artery stents
1．2 Endothelium function mimicking surface modification
1．2．1 ECs functions
1．2．2 Heparin based surface modification
1．2．3 Nitric oxide based surface modification
1．3 Progress of Polydopamine Coating in Biomedical Field
1．3．1 Coating mechanism，structure and preparation
1．3．2 Applications in biomedical field
1．4 Significance and Objectives，Dissertation Layout and Technical route
1．4．1 Significance and objectives
1．4．2 Dissertation layout
1．4．3 Technical route
Chapter 2 Mussel-inspired Chemistry for Biocompatible and Functional Coatings Rich in Amine groups
2．1 Introduction
2．2 Experimental Section
2．2．2 Characterization of the PDAM／HD copolymet coating
2．2．3 HUVEC culture，staining and proliferation
2．2．4 Macrophages evaluation and in vivo tissue response test
2．2．5 Heparin conjugation and quantitative determination
2．2．6 APTT assay
2．2．7 Statistical analysis
2．3 Results analysis
2．3．1 FTIR，amine group density and thickness of PDAM／HD coating
2．3．2 XPS，SIMS results
2．3．3 AFM and WCA results
2．3．4 Mechanical behavior and stability
2．3．5 Adhesion，proliferation and staining of endothelium cell
2．3．6 Macrophages evaluation and tissue response
2．3．7 Conjugation of heparin and bioactivity assay
2．4 Discussion
2．5 Conclusion
Chapter 3 Heparinized Mussel-inspired Coating Baring Amine Group for Multi-Functionalization of Vascular Stent
3．1 Introduction
3．2 Experimental Section
3．2．1 Preparation of PDAM／HD coating
3．2．2 Characterization of PDAM／HD coating
3．2．3 Heparin conjugation and quantification
3．2．4 Hemocompatibility evaluation
3．2．5 Endothelium cell evaluation
3．2．6 Smooth muscle cell evaluation
3．2．6 Competitive Adhesion of ECs and SMCs
3．2．7 In vivo Subcutaneous Implantation Studies
3．2．8 Statistical analysis
3．3 Results analysis
3．2．1 Characterization of Hep-PDAM／HD
3．3．2 Hemocompatibility evalution
3．3．3 Endothelial cell evalution
3．3．4 Smooth muscle eell evalution
3．3．5 Competitive Adhesion of ECs and SMCs
3．3．6 In vivo Subcutaneous Implantation Studies
3．4 Discussion
3．5 Conclusion
Chapter 4 Biomimetic Design of NO-Generating Coatings for Improving the Performance of Vascular Stent
4．1 Introduction
4．2 Experimental Section
4．2．1 Preparation of PDAM／HD Coating
4．2．2 S-S and Se-Se Conjugation
4．2．3 Preparation of PDAM@Cu coatings
4．2．4 Characterization ofthe Coatings
4．2．5 NO Catalytic Release
4．2．6 Hemocomoatibility Evaluation
4．2．7 Smooth muscle cell evaluation
4．2．8 Endothelium cell evaluation
4．2．9 Macrophage evaluation and In vivo animal tests
4．2．10 Statistical Analysis
4．3 Results and discussion
4．3．1 Characterization of S-S@PDAM／HD and Se-Se@PDAM／HD Coatings
4．3．2 NO Catalytic Release
4．3．3 Hemocompatibility Evaluation
4．3．4 Characterization of PDAM@Cu coatings
4．3．5 NO Catalytic Release
4．3．6 Hemocompatibility and enhanced cGMP expression
4．3．7 Endothelium cell evaluation
4．3．8 Marophage Evaluation and tissue response
4．4 Conclusion
Chapter 5 Summary and Outlook
5．1 Summary
5．2 Outlook
Acknowlegements
References
Abbreviation List
Scientific achievements
