文摘
英文文摘
文献综述第一章植物抗病(R)基因介导的抗病反应分子机制
1植物抗病性的划分
1.1非寄主抗性(non-host resistance)
1.2非小种专化性抗性(race non-specific resistance)
1.3小种专化性抗性(race specific resistance)
2已经克隆并预测蛋白结构的植物R基因的类型及结构特点
3R基因介导的植物抗病反应分子机制
3.1 R基因介导的植物—病原特异识别
3.2 R蛋白诱导的HR及早期局部信号传递网络
3.3植物抗病反应中的系统信息作用网络
参考文献
文献综述第二章控制大麦白粉病抗性的基因及其抗性分子机制研究进展
1广谱(broad-spectrum)mlo抗性
1.1 mlo抗性表型
1.2 mlo突变体的多效性影响
1.3 mlo抗性所需的基因
1.4 Mlo蛋白家族
1.5 Mlo蛋白分子功能
1.6大麦野生型Mlo基因的生物学功能
2小种专化性抗性
2.1小种专化性抗性表型
2.2 Mlalocus的分子分析
2.3 Rar1基因为小种专化性抗性所必需
2.4St1基因为小种专化性抗性所必需
2.5 Rar1和Sgt1在疾病抗性中的分子功能
参考文献
文献综述第三章控制小麦抗白粉病抗性的基因及其抗性机制研究进展
第一节小麦抗白粉病基因的来源、染色体定位及分子标记
1 Pm基因的来源
2 Pm基因染色体定
3 Pm基因的分子标记
第二节小麦白粉病抗性机制研究进
1小麦白粉菌致病的三个环节
2小麦针对白粉菌的防御策略
3小麦白粉病抗性基因Pm3b的克隆及对其抗病机制的预测(对单个基因的研究)
4小麦抗白粉病相关基因的研究(从基因群体水平上研究)
参考文献
研究结果第一章小麦与白粉菌亲和及非亲和互作早期H2O2反应的比较分析Comparative analysis of early H2O2 defense in compatible and incompatible wheat-powdery mildew interactions
Summary
Introduction
Materials and Methods
Plants, pathogens and inoculation
Histochemical detection of H2O2 at interaction sites
Results
H2O2 accumulation in response to PGT and AGT in wheat-powdery mildew compatible and incompatible interaction
H2O2 in incompatible interaction mediated by the Pm2+Pm6 genes
H2O2 in incompatible interaction mediated by the Pm16 gene
H2O2 in compatible interaction mediated by susceptible lines
Discussion
Acknowledgements
References
研究结果第二章瞬时表达体系的构建
第一节一个用于白粉病菌侵染大麦或小麦叶片早期反应基因功能分析的瞬时表达体系A transient expression system for the functional assessment of early response genes on the powdery mildew infected barley or wheat leaves
1 The principle and the key steps of the transient assay system used in the interaction between barley or wheat and the powdery mildew
2 The development of the transient assay system and its application
3 The prospect of the transient assay system in the functional assessment of early response genes on the powdery mildew infected barley or wheat leaves
Acknowledgements
References
第二节应用HeliosTM基因枪将外源基因导入小麦完整叶片Gene delivery into intact wheat leaves using the HeliosTM gene gun
Introduction
Materials and methods
Results and discussion
Conclusion
References
研究结果第三章白粉菌诱导下小麦早期反应基因的筛选及功能分析Identification and functional assessment of early response wheat genes induced by powdery mildew
Abstract
Introduction
Materials and methods
Plants, pathogens and inoculation
Chemical Induction and leaves harvested
cDNA-AFLP
Semi-quantitative RT-PCR analysis of transcripts
Generation of full length clones
Transient transformation assay
Results
Identification of wheat genes induced by powdery mildew fungus
Validation of expression patterns by semi-quantitative RT-PCR analysis
Isolation ofa wheat cDNA encoding a CDPK
TaCDPK2 over expression
Differential expression of TaCDPK2 in different tissures and upon abiotic stress
Isolation of a wheat cDNA encoding aperoxinredoxin Q
TaPrxl over expression
Differential expression of TaPrxl in different tissures and upon abiotic stress
Isolation ofa wheat cDNA fragment with homology to auxin response factor 2 from rice
Discussion
References
研究结果第四章小麦脂质转运蛋白的cDNA的克隆及功能分析cDNACloningand FunctionalAnalysis of Lipid Transfer Protein of Wheat
1 Materials and Methods
1.1 Plants and pathogens
1.2 Different treatments
1.3 Total RNA isolation and cDNA synthesis
1.4 Amplification, recovering and cloning of LTP full-length cDNA
1.5 DNA sequence analysis and clustal analysis
1.6 Semi-quantitative RT-PCR analysis of transcripts
1.7 Transient expression analysis
2 Results
2.1 Isolation of full-length cDNA encoding LTP
2.2 The comparison analysis of the coding region of LTP in A, S, AB, D and ABD genome
2.3 Differential expression of LTP (LTP1+LTP2) upon powdery mildew fungus infection
2.4 Differential expression of LTP (LTP1+LTP2) upon abiotic stress
2.5 Differential expressions of LTP (LTP1+LTP2) in different tissues
2.6 LTP1 over expression
Discussion
References
致谢
附录
