Evaluation of Removal Ability for Hexavalent Chromium by Marine Microorganism

摘要

Bioremediation is attractive provision for soil pollution by heavy metal. In this study, focusing on Hexavalent chromium (Cr (VI)) whose elution was confirmed from polluted soil to groundwater in Tokyo, Alteromonas macleodii which is marine microorganism was used for bioremediation of Cr (VI). This study aimed to reveal culturing conditions to obtain maximum removal of Cr (VI) by Alteromonas macleodii. The microorganism was cultivated in medium containing Cr (VI), and culturing conditions included various pH (6,7,8), temperature (15,20,25℃), and concentration of Cr (VI) (1,10,40,100 mgCr (VI)/L). After 24 hour cultivation, removal of microorganism in culture by centrifugal machine was conducted, and Cr (VI) concentration was measured. As a result, pH and temperature had no effect on removal ability of Cr (VI) by Alteromonas macleodii. This study revealed that A heromonas macleodii could remove Cr (VI) whose concentration was 10mgCr (VI)/L, which is the optimum culturing conditions. But, it was difficult to remove Cr (VI) whose concentration exceeded 10mgCr (VI)/L. After 24 hour cultivated, Cr (VI) concentration was 0.5mgCr (VI)/L when culture conditions was 10mgCr (VI)/L. Moreover, it was attested that removal ability was harmonized with microbial concentration.