Plant-based technologies to enable rapid response to Ebola outbreak

摘要

1. Two recombinant vaccine candidates based on Ebola surface GP were expressed in N. benthamiana and purified as enveloped and non-enveloped VLPs and their in vivo efficacy demonstrated. 2. Rapid production of three Ebola monoclonal antibodies in N. benthamiana was demonstrated and in vivo protective activity was confirmed. 3. Major advantages of plant-based expression systems include: 3.1 Ability to produce large quantities of target proteins at low cost 3.2 The presence of an eukaryotic post-translational modification machinery 3.3. Low risk to introduce human pathogens 3.4. Plant tissues can be inexpensively processed for oral delivery 4. The results warrant further development of a novel plant-based vaccines and biologics.