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Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique

机译:利用计算机屏幕光辅助技术的二维微阵列荧光指纹图谱

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摘要

Detection and classification of fluorescent dyes are demonstrated using a computer screen photo-assisted technique (CSPT). This technique has previously been demonstrated for analyzing fluorescence from 96 wells microtiterplates (200 μl per well) and from a single cuvette with some optics to enhance sensitivity. In this work a custom designed array of wells with a volume of approximately 1 μl is used. In order to measure such small volumes without saturating the detector, the transmitted light is masked by placing the sample between two crossed polarizers. This arrangement blocks nearly all the transmitted light, while the emitted light, which is nearly unpolarized, can still be detected. The lowest amount (concentration x volume) of analyte detectable in this setup is about 40 times smaller than in the previous setups.
机译:使用计算机屏幕光辅助技术(CSPT)证明了荧光染料的检测和分类。先前已经证明了该技术可用于分析96孔微量滴定板(每孔200μl)和单个比色皿的荧光,并带有一些光学器件以增强灵敏度。在这项工作中,使用定制设计的孔阵列,其体积约为1μl。为了在不使检测器饱和的情况下测量如此小的体积,可以通过将样品放置在两个交叉的偏振器之间来掩盖透射光。这种布置几乎阻挡了所有的透射光,而仍然可以检测到几乎无偏振的发射光。此设置中可检测到的最低分析物量(浓度x体积)比以前的设置小约40倍。

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