Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique

摘要

Detection and classification of fluorescent dyes are demonstrated using a computer screen photo-assisted technique(CSPT). This technique has previously been demonstrated for analyzing fluorescence from 96 wells microtiterplates (200 μlper well) and from a single cuvette with some optics to enhance sensitivity. In this work a custom designed array of wellswith a volume of approximately 1 μl is used. In order to measure such small volumes without saturating the detector, thetransmitted light is masked by placing the sample between two crossed polarizers. This arrangement blocks nearly all thetransmitted light, while the emitted light, which is nearly unpolarized, can still be detected. The lowest amount(concentration x volume) of analyte detectable in this setup is about 40 times smaller than in the previous setups.