Analysis Of Carbohydrate Modifications Of Proteins On Sams Surfaces As Motifs For Directing Dendritic Cell Phenotype

摘要

Dendritic cells (DCs) recognize and respond to a foreign object (usually a pathogen) using conserved structures that are characteristic of pathogens and not found in eukaryotic cells or through carbohydrate structures characteristic of pathogens or through op-sonizing proteins. These motifs are named 'pathogen associated molecular patterns' (PAMP), and their cognate binding receptors on the phagocytes, pattern recognition receptors (PRRs) (1). PRRs include, among others, Toll-like receptors (TLRs) (2) and C-type lectins (3). DCs have been shown to undergo maturation upon bio-material contact to an extent depending on the biomaterial used (4). We hypothesize that DCs recognize and respond to biomaterials using these same PRRs either indirectly through the adsorbed protein layer (specifically carbohydrate modifications) or through carbohydrates inherent in the biomaterial chemistry to initiate an innate immune response. To understand the carbohydrates associated with proteins adsorbed to biomaterial surfaces, an enzyme linked lectin assay (ELLA) was used to characterize carbohydrates associated with proteins adsorbed to model self-assembled monolayers (SAMs) of alkanethiols of defined chemistries. The goal is to correlate the range of carbohydrate profiles associated with SAM surfaces with the resultant DC phenotype when they are cultured on these surfaces.