Effect of Cell Ratio on Osteoclast and Osteoblast Differentiation in a Ceramic Bone Substitute System

摘要

Osteoclast and osteoblast synchronization enables bone repair in vivo; hence, co-culture evaluations of implants and bone substitute materials designed for pathological fractures should be performed accordingly. The results of this study suggest that the RAW:D1 ratio of 1:10 in OS medium was more similar, in key aspects, to native conditions than the other culture conditions studied. Most importantly, the results demonstrated that the RAW:D1 ratio selection will influence information gleaned from an in vitro system. Future studies will focus on simulating pathological conditions with varying cell ratios.