The Use of Alkanethiols and Circular Dichroism to Monitor Conformational Changes in Adsorbed Proteins

摘要

The use of SAMs formed on gold-coated quartz slides in CD studies is a feasible method of designing model surfaces that can be used to study protein-surface interactions as a function of surface chemistry. CD with g-Factor analysis offers a reliable method for determining the secondary structure of adsorbed proteins. Using this technique, it is possible to quantify the degree of structural change that occurs at the protein-biomaterial interface.