Small-Interfering RNA-Eluting Surfaces as a Novel Concept for Intravascular Local Gene Silencing

摘要

This study provides the proof of principle of a local gene-silencing strategy that uses surface coatings composed of gelatin, PEI and specific siRNA by which a highly significant knockdown of the E-selectin receptor in human primary endothelial cells was achieved. The E-selection knockdown was around 70%, without a significant reduction of the cell viability. The transfection efficiency was not inhibited by the presence of serum, which is an important prerequisite for intravascular applications.