Interaction of nimodipine with DNA investigated by electrochemical methods

摘要

The electrochemical behavior of nimodipine and the interaction between nimodipine and calf thymus DNA were investigated by cyclic voltammetry and differential pulse voltammetry. It was shown that nimodipine exhibited an irreversible electrochemical process in 0.50 mol L-1 ammonia buffer (pH 8.8) at glassy carbon electrode. The anode peak current decreased with increasing concentration of calf thymus DNA and the formal potential shifted negatively, indicating that the interaction between nimodipine and calf thymus DNA was mainly via electrostatic mode and formed a non-electroactive supramolecular compound. The molar binding ratio and association constant of nimodipine with calf thymus DNA were calculated to be 2 and 9.8 × 10~6, respectively.