SENSITIVE DETECTION OF MICRORNAS WITH SIMPLIFIED LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)

机译：简化的环介导的等温扩增（LAMP）灵敏检测微RNA

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A simplified loop-mediated isothermal amplification (LAMP) system has been designed and investigated for sensitive detection of miRNA. With the simplified LAMP reaction, as low as 1. 0 pM miRNA can be quantitatively detected with one-step operation and the dynamic range spans over 4 orders of magnitude. The LAMP-based miRNA assay also exhibits high selectivity to clearly discriminate one-base difference among miRNA sequence.

机译：已设计并研究了一种简化的环介导的等温扩增（LAMP）系统，用于miRNA的灵敏检测。通过简化的LAMP反应，只需一步操作即可定量检测低至0 pM miRNA，动态范围跨越4个数量级。基于LAMP的miRNA分析还具有很高的选择性，可以清楚地区分miRNA序列之间的一碱基差异。

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来源
《Progress on post-genome technologies and modern natural products》|2011年|384-386|共3页
会议地点 Chongqing(CN)
作者
Li Zhengping; Liu Xiaoling; Li Cuiping; Liu Ye; Li Yankun;
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作者单位

Key Laboratory of Medicine Chemistry and Molecular Diagnosis) Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China;

Key Laboratory of Medicine Chemistry and Molecular Diagnosis) Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China;

Key Laboratory of Medicine Chemistry and Molecular Diagnosis) Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China;

Key Laboratory of Medicine Chemistry and Molecular Diagnosis) Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China;

Key Laboratory of Medicine Chemistry and Molecular Diagnosis) Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China College of Environmental Science and Engineering, North China Electric Power University;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
关键词
Simplified; Loop-mediated isothermal amplification (LAMP); microRNA;

机译：简化环介导的等温扩增（LAMP）;微小RNA;

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专利

1. Asymmetric polymerase chain reaction and loop-mediated isothermal amplification (AP-LAMP) for ultrasensitive detection of microRNAs [J] . Ruili Wang, Ling Lan, Li Liu, 中国化学快报（英文版） . 2020,第001期

机译：不对称聚合酶链反应和环介导的等温扩增（AP-LAMP）用于microRNA的超灵敏检测
2. Rolling circle extension-actuated loop-mediated isothermal amplification (RCA-LAMP) for ultrasensitive detection of microRNAs [J] . Tian Weimin, Li Pengjie, He Wenli, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2019,第期

机译：滚动圈延伸 - 致动环介导的等温扩增（RCA-LAMP），用于微润松检测
3. One-step ultrasensitive detection of microRNAs with loop-mediated isothermal amplification (LAMP) [J] . Cuiping Li, Zhengping Li, Hongxia Jia, Chemical Communications . 2011,第9期

机译：通过环介导的等温扩增（LAMP）一步一步检测microRNA
4. Early Detection of Leptospirosis in Cattle Urine Samples by Using Loop-Mediated Isothermal Amplification (LAMP) Method [C] . Dyah Ayu Widiasih, Heru Susetya, Rini Widayanti International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering . 2018

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SENSITIVE DETECTION OF MICRORNAS WITH SIMPLIFIED LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)

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