Preparation and Characterization of Immobilized Oil-degrading Strain Based on Embedding Method

摘要

The objective of the study was to prepare the immobilized microspheres with embedding method including the chitosan encapsulation, alginate-chitosan encapsulation, PVA-Na2SO4 and PVA-H3BO3 embedding in order to determine the optimized preparation conditions with the bacteria SM-3 as the embedding object and oil degradation rate as the detection index. The results showed that the oil degradation rate of calcium alginate-chitosan microspheres was only 12.49%, which was poorer than the free bacteria in the degradation of 31.12%. And the oil degradation rate of chitosan microspheres, PVA-H3BO3 microspheres, PVA-Na2SO4(starch) microspheres, and PVA-Na2SO4(active carbon) microspheres were 55.73%, 36.02%, 53.18%, and 58.25% respectively, which were all better than the degradation effect of free bacteria. In addition, the performance of various microspheres tests showed that these types of immobilized microspheres had both good flexibility and permeability.