An Integrated Microfluidic Device for Cell-Based High Content Screening

摘要

An integrated microfluidic device was described for cell-based high content screening (HCS), which enabled the multiparametric measurement of cellular responses in human liver carcinoma (HepG2) cells in response to several compounds in a high throughput format. This device consists of multiple drug gradient generators and parallel cell culture chambers, in which the processes of liquid dilution, micro-scale cell culture, cell stimulation and cell labeling can be integrated into a single device. This assay provides rich information involving plasma membrane permeability, nuclear size and mitochondrial transmembrane potential in anti-cancer drug-induced apoptosis of HepG2 cells, demonstrating the potential of this platform for application in the early stage of drug discovery.