Evaluating Ganoderma lucidum Strains for the Production of Bioactive Components

摘要

In the present studies, mycelial biomass of Ganoderma lucidum strains was produced in Mushroom Complete Media under optimized culture conditions of temperature, pH, agitation and the fruiting bodies were raised on wheat straw supplemented with 5 per cent rice bran. Maximum biological efficiency of 13. 1 per cent was obtained in 57 days. Polysaccharides and triterpenes were extracted, characterized and analysed. The maximum yield of polysaccharides was obtained from fruiting bodies (30. 06%-63. 70%), followed by mycelial biomass (7.61%-21.32%) and culture filtrates (0.86%-2.86%). Non-reducing sugars were the main fraction of sugars which constituted 69. 82-70. 39 per cent in fruiting body extracted polysaccharides, 62. 08-83. 39 per cent in biomass and 59. 75-77. 54 per cent in crude extracts. Triterpenes were separated on the basis of R1 using TLC and characterized using mass spectrometry which revealed the presence of many types of triterpenes (Ganoderic acid A, AM-1, B, J, H, Lucidenic acid N & A, Ganolucidic acid A & D and two new triterpenes 3 (3-hydroxyl-4,4,14-trimethyl-7,11 ,15-trioxochol-8en-24-oic acid and 7, 15-dihydroxy-4, 4, 14trimethyl-3, 11-dioxochol-8-en-24-oic acid). The extracts from fruiting bodies and biomass of G. lucidum strains also exhibited antibacterial effect against some pathogenic bacteria, Staphylococcus sp. and Enterobacter sp.