Biosensing Systems Based on Metal Oxides Nanoparticles and Choline Oxidase for Environmental and Biomedical Monitoring of Neurotoxicants

摘要

In this study we investigated the effects of monovalent anions in the series F~-, Cl~-, Br~-, I~- on the sensitivity of the biosensor assembled from polyca-tions and ChO via theirs layer-by-layer deposition on the surface of carbon electrodes (CE) coated with MnO_2. The efficiency of gamma-phase-MnO_2 nanoparticles, deposited on screen-printed electrodes, as a new transducer element of biosensing platforms, fabricated with "layer-by-layer" technology was demonstrated. The present report describes analytical possibilities of the biosensing platform. The hydrogen peroxide registration and subsequent application for neurotoxicants assay in the environmental field. The validation of peroxide-sensitive sensors, as a new transducer element based on MnO_2 nanoparticles, for fabrication of choline oxidase biosensor has been done. The revealed analytical characteristics of the choline oxidase biosensor are perspective for highly-sensitive monitoring of cholinesterases and their inhibitors in medical, biological and environmental samples.