Characterization of rat liver mitochondria using time-resolved spectroscopy

摘要

Abstract: In this study, the $mu$-s$/$PRM (scattering constant) and the $mu$-a$/ (absorption constant) of rat liver mitochondria were measured by time resolved spectroscopy (TRS) with a newly innovated medium matching method. Freshly isolated rat liver mitochondria were placed inside a pouch and submerged in an intralipid emulsion. Two sets of various concentrations of intralipid emulsion, one with different concentrations of intralipid and no ink and another with various concentrations of ink and a fixed intralipid concentration, were scanned for matching the $mu$-s$/$PRM and the $mu$-a$/ of the mitochondria. Two states of the mitochondria were scanned: the resting state (orthodox state, or state 4) and the active state (condensed state, or state 3). During the active state of the mitochondria, the active respiration of the mitochondria was initiated by the addition of either calcium chloride or ADP/succinate/P$-i$/. Comparisons of the $mu$-s$/$PRM and the $mu$-a$/ were made of mitochondria at different states. !11