Immobilized Fluorescent Liposome Column for Bioanalysis and Signal Amplification

摘要

Fluorescent dye, calcein-entrapped unilamellar liposomes were stably immobilized in gel beads by avidin-biotin binding to construct the stationary phase for immobilized liposome chromatography (ILC). High-immobilized amounts, excellent stability and membrane integrity of the immobilized liposomes provide the basis for their applications in bioanalysis and biotechnology. This chapter reports the following subjects using the immobilized fluorescent liposome column: (1) Sensitively analysis interactions between the drugs, lipophilic cations, peptides and proteins with lipid bilayers. Retardation of solutes was monitored using a UV detector; meanwhile perturbation of the membranes by some solutes gave rise to the calcein leakage monitored by an online fluorescent detector. (2) Rapidly detection of Phospholipase A_2 (PLA_2)-catalyzed membrane leakage. (3) Detection of small molecules using a competitive immuno-reaction and sensitization by PLA_2-catalyzed dye leakage from immobilized liposomes.