New optoelectronic system based on the surface plasmon resonance phenomenon: application to the concentration determination of DD-fragment of fibrinogen

摘要

Abstract: The interaction of DD-fragment of fibrinogen withantibodies immobilized on the surface ofpolycrystalline gold films monoclonal has been studiedby means of Surface Plasmon Resonance (SPR) biosensorinstrument as a model system for real-time biomolecularinteraction analysis. A BioHelper Plasmon-002 device(ISP NASU, Kiev, Ukraine), that exploits the SPRphenomenon to detect the interactions between sensorlayer and analyte has been used for investigation. Theresults show that the kinetic parameters ofantigen-antibody interactions are directly related toconcentration of DD- fragment of fibrinogen in theexamined solutions in the range from 10$+2$/ to 10$+5$g/ml. The binding kinetic data can be described by aset of several time-division binding equilibrium, eachof them correlated well with an ideal bindingfirst-order reactions. The influence of the procedureof antibody immobilization on the quasi flat goldsurface has been examined as well.!20