Optical coherence tomography for ultrahigh-resolution 3D imaging of cell development and real -time guiding for photodynamic therapy

摘要

Optical coherence tomography is a new emerging technique for cross-sectional imaging with high spatial resolution of micrometer scale. It enables in vivo and non-invasive imaging with no need to contact the sample and is widely used in biological and clinic application. In this paper optical coherence tomography is demonstrated for both biological and clinic applications.rnFor biological application, a white-light interference microscope is developed for ultrahigh-resolution full-field optical coherence tomography (full-field OCT) to implement 3D imaging of biological tissue.rnSpatial resolution of 0.9umxl.lum (transverse × axial) is achieved A system sensitivity of 85 dB is obtained atrnan acquisition time of 5s per image. The development of a mouse embryo is studied layer by layer with our ultrahigh-resolution full-filed OCT.rnFor clinic application, a handheld optical coherence tomography system is designed for real-time and in situ imaging of the port wine stains (PWS) patient and supplying surgery guidance for photodynamic therapy (PDT) treatment. The light source with center wavelength of 1310nm, -3 dB wavelength range of 90 nm and optical power of 9mw is utilized. Lateral resolution of 8 um and axial resolution of 7um at a rate of 2 frames per second and with 102dB sensitivity are achieved in biological tissue. It is shown that OCT images distinguish very well the normal and PWS tissues in clinic and are good to serve as a valuable diagnosis tool for PDT treatment.