Differential Effects of Surfactants on Enzyme Activity and Transport across a Semipermeable Cell

摘要

This study systematically investigated how anionic surfactants of various hydrophilicities affected the activities of three metabolically important enzymes — glutamate dehydrogenase (GDH), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) — of different molecular masses at a pH range important to body functions (6.5-7.4). We also investigated the time course of the surfactant concentration dependent effects on LDH. To observe how surfactants would affect the cellar protein transport, a separation cell with a semipermeable membrane was used to simulate a passive mass transport phenomenon between cells, with various concentration combinations of enzymes and surfactants.