Electrochemical Evaluation of the Properties of the Immobilized Enzyme Glncose Oxidase

摘要

The enzyme glucose oxidase was evaluated as a recognition element in electrochemical biosensors. Immobilization techniques as physical adsorption, entrapment in electrogenerated polymer film, affinity and covalent binding are discussed based in the involved physicochemical and kinetic phenomena. This includes the determination of the main kinetic parameters: apparent Michaelis-Menten constant K_m~(app) and maximal rate I_(max) of the heterogeneous enzyme reactions of glucose oxidation in the absence and in the presence of inhibitors. Parameters as pH, optimal temperature and stability of the biocatalyst were established analyzing the transfer function of the developed amperometric glucose oxidase sensors of first and second generationrnI=I_(max)[S]/(K_m~(app)+[S]).