Acute Phase Protein Serum Amyloid A (SAA) Profile in Diabetic Wistar Rats Induced Streptozotocin

摘要

Diabetes mellitus is a disease caused by the destruction of pancreatic β-cells that leads to the loss of insulin secretion. Diabetes mellitus is associated with an inflammatory state characterized by the appearance of acute phase protein. In mammals, one of the dominant acute phase proteins is Serum Amyloid A (SAA). This study aims to determine acute phase protein SAA levels and its correlation with blood glucose levels in diabetic rats induced with streptozotocin so that it can be used as biomarkers of diabetes mellitus. Rats used were 20 male Wistar rats, two months old and weight 180 g to 250 g. Rats were divided into two groups, each group consisting often rats which group I as treatment and group II as a control. Group I was fasted for 24 h and then injected 1 mg streptozotocin once at doses 40 mg ? kg~(-1) BW dissolved in 0.1 M sodium citrate buffer pH 4.0. The rat's blood was being collected at (0~(th), 6~(th), 12~(th), 24~(th), 36~(th), 48~(th), 60~(th), 72~(nd), 84~(th), and 96~(th)) h of post diabetic induction for blood glucose and SAA protein examination. The results showed that Serum Amyloid A (SAA) protein levels had a positive correlation with blood glucose levels in diabetic rats induced with streptozotocin so that SAA can be used as biomarkers in diabetic rats especially at 24 h to 60 h post induction.