Using Zebrafish Embryo to Establish a Test Method to Verify Toxicity of Dibutyl Phthalate(DBP)

摘要

Dibutyl phthalate (DBP) is a commonly used plasticizer.In laboratory experiments,it has been shown to cause developmental defects,and changes in the testes and prostate,and reduced sperm counts.In this study,zebrafish embryos are model organisms which were tested DBP influence in development.In DBP toxicity testing,zebrafish embryos exposed DBP in different period.Respectively,LC50 in 24,48 and 72 hours was 4.04ppm,1.85ppm and 0.95ppm.After experiment exposured,zebrafish embryos that were hatched successfully could observe enlarged yolk sac,spinal curvature,uneven skin pigmentation and yolk-sac malformation on the larvae.The results indicated that adverse effects of low concentration DBP could detect by zebrafish embryo and this test method has high sensitivity.The all results show that embryonic was exposed in DBP environment,could be caused unnormal embryonic development.Using zebrafish embryos acute toxicity test to identify DBP concentration range in water.In the future,it can be an indicator to assess the pollution of environment DBP.