Isolation, Characterization, in Vitro and in Silico Antidiabetic Activity of Bioactive Compound of Katemas Leaves (Euphorbia Heterophylla, L.)

摘要

The purpose of this study is to isolate, purify and characterize active compounds from E. heterophylla L. plants and to know the antidiabetic activity of pure Katemas (E.heterophylla, L.) leaf compounds in vitro and in silico (molecular docking) through inhibition of alpha glucosidase enzymes. In this study sample extraction was carried out using multilevel maceration, starting with n-hexane, then with ethyl acetate. Isolation was done by VLC. Antidiabetic activity was tested for pure compounds through inhibition of the α-glucosidase enzyme in vitro using a microplate reader and in silico (molecular docking) using Discovery Studio 4.1 software. VLC results from the ethyl acetate extract of katemas leaves obtained 21 fractions and taken f2 because of the potential for more and stable crystals. F2 is predicted to be a DS-01 diterpenoid compound according to the results of UV, IR, H~1-NMR, C~(13)-NMR and MS spectroscopy. The results of antidiabetic activity test in vitro obtained IC_(50) values of 138.63 μg/mL for ethyl acetate extract and IC_(50) values of 191.82 μg/mL for diterpenoid DS-01 compounds. The results of molecular docking showed that the active compounds contained in the extract and also DS-01 diterpenoids were able to form hydrogen bonds between the ligand with the receptor, but less when compared with the acarbose.