(Invited) Eight Channel Microphysiometry Using a Lab on a Chip Microclinical Analyzer

摘要

We have built a multianalyte microphysiometer that detects multiple analytes involved in the cellular bioenergetics simultaneously. Monitoring the uptake of glucose and oxygen, and the production of lactate and acid gives a more complete picture of the metabolic processes within the cell than just acidification microphysiometry alone. Metabolic processes such as glycolysis, mitochondrial ATP generation, and glycogenesis are all directly related to the flux of these analytes. Temporal resolution of metabolic responses is much faster than conventional well-plate studies, leading to dynamic metabolic data. Electrochemical sensors are easily adapted into microfluidic formats for microphysiology readouts.