Studies on the Mechanism of Electrochemical Detection of Haemophilus Influenzae at Diamond-Based Electrodes

摘要

Haemophilus influenzae (HI) is a Gram-negative pathogenic bacterium whose infections occur worldwide and mainly affect unvaccinated children and those who have not completed the basic vaccination course. Type B of this bacterium (Hib) can cause sepsis, and other invasive diseases as dermatitis or arthritis but the most dangerous is pneumonia meningitis. The most reliable method for recognizing Hib infections is isolation in microbiological culture. Serological tests are also performed to diagnose Hib infections. They rely on the detection of the presence of bacterial antigens or antibodies which have the ability to bind to specific antigens. Detection of antibodies doesn't mean disease but only indicate contact with a given bacterium in the past. Recognition of bacteria using presently known methods takes a long time and requires expensive equipment although fast and sensitive detection of Haemophilus influenzae is crucial 1 to start treatment early enough.