(Invited) Development of a Single Walled Carbon Nanotube Sensor for Long Term Implantation and Detection in a Large Animal Model

摘要

Fluorescent sensors are frequently not used for in vivo research due to photobleaching and difficulty in signal detection. Single walled carbon nanotubes (SWNT) circumvent these issues with near infrared fluorescent emission and long term stability; yet detection of carbon nanotube sensors has never before been performed in large animal models. The ability to place and read SWNT sensors in vivo for a large animal requires specialized instrumentation and research facilities. By teaming up with large animal veterinarians and meat scientists, our lab was able to surgically place, monitor, and recover SWNT sensors from 14 sheep. We examined various SWNT platforms, implantation locations, and detection methods to determine a strategy for frequent, consistent detection of SWNT sensors that serve as an indicator of animal stress and overall health.