Nanopore represents a unique and versatile sensor to analyze and manipulate single entities. We are aiming to develop a single-entity analysis platform based on the elegant electrochemically confined nanopores. The key ideal is confining the enhanced electric field within the narrow aperture of nanopore to analyze functional and dynamic information of single molecule, single nanoparticle, and single cell. Volume exclusion effect was firstly coupled with surface charge effect to track the dynamic process of Amyloid-β Aggregation. Then, we employed the confined space of nanopipette as a nano-reactor and sensing region to form a silver nanoparticle (Ag NP) electrode, and to successfully monitor dynamic process of hydrogen evolution reaction (HER) catalyzed by MoS_2 quantum dots (MoS_2 QDs). Furthermore, nanopipette with electrochemically confined effect also acts as nanosyringe to attract cell inclusion and deliver fluorescent molecule to a single cell. As a result, a single cell manipulating was successfully achieved.
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