Identification of new autoantigens in Multiple Sclerosis by glycopeptide libraries

摘要

The glycopeptide CSF114(Glc)is the first synthetic antigen able to detect specific auto-antibodies(auto-Abs)in solid-phase not competitive indirect ELISA on sera of patients affected by Multiple Sclerosis(MS).We demonstrated that the antibody titre correlates with the disease activity.CSF114(Glc)displayed a well-defined conformational preference in solution characterized by a p-hairpin motif,with the minimal epitope Asn(Glc),fundamental for the auto-Ab recognition,on the tip of a type I' beta-turn structure.